Balto and his mate Jenna have a new family of six puppies. Five of their puppies look like their husky mother, while one pup named Aleu clearly takes her looks from her wolfdog father. When they all reach eight weeks old, all of the other pups are adopted to new homes, but no one wants Aleu. Aleu stays with her father, Balto. A year later, after she is almost killed by a hunter, Balto tells Aleu the truth about her wolf heritage. In anger and sadness, she runs away, hoping to find her place in the world. At the same time, Balto has been struggling with strange dreams of a raven and a pack of wolves and he cannot understand their meaning. When Aleu doesn't come back the next day, he runs off to find her and bring her back home. He meets with mysterious creatures, like a cunning fox, a trio of wolverines that taunt him, the same guiding raven from his dreams, and a furious grizzly bear that suddenly disappears as if it was never there. During the journey, his friends Boris, Muk and Luk hope to find Balto, but they are halted by some unknown force. They soon realise that this journey to find Aleu is meant for the father and daughter themselves. Aleu, after taking refuge in a cave, meets the field mouse called Muru who lets Aleu realise that being part-wolf isn't so bad. He teaches her that everyone has a spirit guide. After singing the song "Who are you?", it turns out that Muru is Aleu's spirit guide. When Aleu and Balto reunite after a close escape from the bear, Aleu realizes she has gained a strange ability that allowed her to see the bear's thoughts. Aleu has started to grow, telling her father that she's not going home until she finds out who she is. The two of them travel onward, both following the raven, to a starving pack of wolves by the ocean. They are led by an old wolf named Nava, who has magic powers and can contact the mysterious white wolf Aniu in his "dream visions". He tells his pack that one day soon, they will be led by a new leader, "the one who is wolf but does not know". Everyone believes that Balto, who is half wolf himself, is the chosen one that Aniu was speaking of. However, Niju (a young wolf), hopes that he will be the next leader since he is stronger and more powerful than the old, wise Nava. He plans to accomplish that with his followers Nuk, Yak and Sumac. The day comes to depart from their home to follow the caribou, the wolves' food source, across the large sea using pieces of ice like a bridge, with Balto in the lead. When Nava is separated from the rest of the pack, Aleu joins him to help him across, but runs into Niju, who is ready to take the elderly leader's life and the young half-wolf's as well. Balto abandons the pack to save his daughter, but before anyone gets hurt, they realise that the pack is floating away, leaderless. Nava cannot make the swim in his old age, so Balto tells Niju to be their new leader and to swim across to the pack, but Niju refuses to leave his homeland. Balto is prepared to help the pack, but Aleu realises that this is where she truly belongs. She makes the swim to the pack to become its leader as Nava returns to his home to find Niju. As Balto makes his way back to Nome, the raven reveals its true form as the great white wolf, Aniu, who is Balto's mother.
- Casper, Wendy, Jazmine Payne, Carly Shay, Spencer Shay, Freddie Benson, Sam Puckett, Gibby, Nigel Baker, Kyle Baker, Mike Baker, Mark Baker, Jessica Baker, Kim Baker, Sarah Baker, Jake Baker, Henry Baker, Lorraine Baker, Cooper, Dylan Schenk, Ben Hilton, Jamie, Max, Becca, Nicky, Jeannie, The Flash/Tony, Crispin, Dylan, Duncan, Sean, Tony's Brother, Juel, Erin, Shirley Temple, The Barney & Friends and the Gang, German Speaking Boy, The Chipmunks (Alvin, Simon, and Theodore), The Chipettes (Brittany, Jeanette, and Eleanor), Aldo, Ethan, Miarissa, Bina, Ely, Otter, Tilly, Tom, Tiny, Ramona, Tina, Teddy, Denise, Derek, Harry, Rusty, Walter, Max, Sharkboy, Lavagirl, Linus, Marissa, Ely, Otter, Bindi, Rosie, Kelly, Lynn, Annie, Molly, Pepper, Duffy, Kate, July, The Fraggle Rock Gang, Andy, Ruby, Gracie, Kyle, Chyna Parks, Olive Doyle, Fletcher Quimby, Lexi Reed, Cameron Parks, Nat Wolff, Alex Wolff, Thomas Batuella, Allie DiMeco, David Levi, Qaasim, Cooper Pillot, Jesse Draper, Michael Jackson & Friends, Michael Jackson Badder, Spanky, Stymie, Froggy, Porky, Buckwheat, Uh-Huh, Waldo, Mary-Ann, Jane, Rascals #1, 2, 3, and 4, Friends of Stymie and Uh-huh, Darla, Alfalfa, Little Carbon Soldiers, Becky O'Shea, Junior Floyd, Jake Berman, Rudy Zolteck, Tad Simpson, Hanon, Marcus, Timmy Moore, Nubie, Johnny Vennaro, Briggs, Patterson, Priscilla O'Shea, Murphy, Keith, Marcus, Hector, Jessica, Lisa, Shock, Pete and Pete, Pablo, Tyrone, Tasha, Uniqua, Austin, Paul, The Oslen Twins, Calvin Cambridge, Murph, Ox, Marlon, Reg Stevens, Max Keeble, Megan, Robe, Kevin McCallister, Alex, Leah, Matilda, Penelope, Matthew, Dwayne, Steve, Robert, Nelly K., Sonny, Tawni Hart, Chad Dylan Cooper, Nico Harris, Grady Mitchell, Zora Lancaster, the Baby-Sitters Club gang, Simba, Nala, Timon, Pumbaa, Baloo, Kovu, Kiara, Uncle Max, Ma, Taran, Princess Eilonwy, Fflewddur Fflam, Gurgi, Jesse Greenwood, Elvis, Randolph Johnson, Nadine, The Goonies (Mikey Walsh, Brand Walsh, Mouth Devereaux, Data Wang, Chunk Cohen, Andy Carmichael, Stef Steinbrenner, and Sloth Fratelli), Scooby-Doo, Shaggy Rogers, Fred Jones, Daphne Blake, Velma Dinkley, Yogi Bear, Boo-Boo Bear, Snagglepuss, Huckleberry Hound, Quick Draw McGraw, Augie Doggie and Doggie Daddy, Wally Gator, Magilla Gorilla, Top Cat, Peter Potamus, Woody Woodpecker, Ttark, Toaster, Lampy, Radio, Blanky, Kirby, Fievel Mousekewitz, Tiger, Tony Toponi, Tanya Mousekewitz, Yasha Mousekewitz, Papa Mousekewitz, Mama Mousekewitz, Charlie Barkin, Itchy Itchiford, Hubie, Rocko, Jack Skellington, King Louie, Genie, Louis, Sebastian, Alex, Marty, Melman, Gloria, King Julien, Maurice, Mort, Roger Rabbit, and The Entire LionKingHeart Fantasy Films team and the rest of the characters will guest star in this film.