In this all-new adventure, Ryan, Alvin, Winnie the Pooh and the rest of the characters met Kermit the Frog for the first time. Together with Fozzie Bear and the rest of the Muppets they head out on a road trip to Hollywood California and avoid being captured by Doc Hopper and Kids World's old enemy: Ursula The Sea-Witch.
- Ursula returns in this film to get revenge on Ryan, Alvin, Pooh and the rest of the gang.
- Ash Ketchum, Pikachu, Misty, Togepi, Brock and the Pokemon gang, Simba, Nala, Timon, Pumbaa, Zazu, Kovu, Kiara, Ma, Uncle Max, Rafiki, Littlefoot, Cera, Ducky, Petrie, Spike, Chomper, Ruby, Guido, Grandpa Longneck, Grandma Longneck and the Land Before Time Gang, SpongeBob SquarePants, Patrick Star, Squidward Tentacles, Sandy Cheeks, Eugene Krabs and the gang, Bloom, Stella, Flora, Musa, Tecna, Layla, Stan Marsh, Kyle Broflovski, Eric Cartman, Kenny McCormick, Jean-Bob, Speed, Puffin, Terk, Tantor, Louis, Ray the Firefly, Genie, Iago, Sebastian, Baloo, Bagheera, King Louie, the Vultures, Jiminy Cricket, Mickey Mouse, Donald Duck, Goofy, Kenai, Koda, Rutt and Tuke, Fu Dog, Roger Rabbit, Woody, Buzz Lightyear, Charlie Barkin, Itchy Itchiford, Jack Skellington, Chanticleer, Owen, Izzy, Bob the Tomato, Larry the Cucumber, Junior Asparagus, Jimmy and Jerry Gourd, the Powerpuff Girls (Blossom, Bubbles, and Buttercup), the Rugrats Gang, Carly Shay, Sam Puckett, Alvin, Brittany, Bobby, Cindy, Oliver, Freddie Benson, Spencer Shay, Gibby, Popeye, Olive Oyl, Popeye's Nephews (Peepeye, Poopeye, Pipeye, and eakey, Jumba, Mario, Luigi, Princess Toadstool, Toad, Yoshi, Donkey Kong, Diddy Kong, SoPupeye), Scrooge McDuck, Huey, Dewey, Louie, Webby, Launchpad McQuack, Chip and Dale, Lilo, Stitch, Plnic the Hedgehog, Miles "Tails" Prower, Knuckles the Echidna, Amy Rose, Cream the Rabbit, Cheese the Chao, Shadow the Hedgehog, Rouge the Bat, Team Chaotix (Vector the Crocodile, Espio the Chameleon, and Charmy the Bee), Silver the Hedgehog, Blaze the Cat, Spyro the Dragon, Sparx the Dragonfly, Banjo and Kazooie, Barney the Dinosaur and the gang, Baby Bop, BJ, Riff, The Griffins (Peter, Lois, Chirs, Meg, Brian, and Stewie), Pocahontas, Meeko, Flit, Princess Aurora, Peter Pan, Tinkerbell, Terrence, Fawn, Rosetta, Silvermist, Iridessa, Vidia, Alice, Hercules, Phlioctetes, Pegasus, Phineas and Ferb, Isabella Garcia-Shapiro, Alex, Marty, Melman, Gloria, The Penguins of Madagscar (Skipper, Kolwalski, Rico, and Private), King Julien, Maurice, Mort, Taran, Princess Eilonwy, Fflewddur Fflam, Gurgi, Jesse Greenwood, Elvis, Randolph Johnson, Nadine, The Goonies (Mikey Walsh, Brand Walsh, Mouth Devereaux, Data Wang, Chunk Cohen, Andy Carmichael, Stef Steinbrenner, and Sloth Fratelli), Aladdin, Princess Jasmine, Abu, Yogi Bear, Boo-Boo Bear, Snagglepuss, Huckleberry Hound, Quick Draw McGraw, Augie Doggie and Doggie Daddy, Wally Gator, Magilla Gorilla, Top Cat, Peter Potamus, Scrooge McDuck, Huey, Dewey and Louie, Webby, Tom, Huckleberry, Becky, Scooby-Doo, Shaggy Rogers, Fred Jones, Daphne Blake, Velma Dinkley, Bugs Bunny, Daffy Duck, Woody Woodpecker, Ttark, Rex, Woog, Elsa, Dweeb, Fievel Mousekewitz, Tiger, Tony Toponi, Tanya Mousekewitz, Yasha Mousekewitz, Papa Mousekewitz, Mama Mousekewitz Simba, Huey, Dewey, Louie, Ash and the characters, Littlefoot and the characters, Spongebob and the characters, Scooby-Doo and his friends, Ronald McDonald and his friends, Ariel, Melody, Princess Jasmine, Belle, Princess Aroura, Princess Odette, Princess Tiana, Snow White, the entire cast from Regular Show, Meta Knight, Pajama Sam, Donkey Kong, Diddy Kong, Dr. Rabbit, Dr. Brushwell, the Star Fox team, the Kids Next Door, Numbuh 362 Kids World's Adventures Team, the Tiny Toons gang, and the rest of the characters are heroes, Blard Simpleton, Henry the lizard, the Bowser family, King Ghidorah, Plankton, Ozzy, Strut, Ichy and Dil, the Star Wolf team, the Predator, One-Eyed Sally, Blather, the Crime Empire, Kaput and Zosky and the Subspace Empire) will guest stars in this film.
- Fozzie Bear is revealed to be Yogi Bear, Pooh, Chito, Rita, and the rest of the 3rd cousin.
- This film features a flashback from Kids World's Adventures of The Little Mermaid which shows Ryan, Pooh and the rest of the gang and their first encounter with Ursula.