The evil emperor Shao Kahn (Brian Thompson) opens a portal from Outworld to the Earthrealm and has reclaimed his queen Sindel (Musetta Vander), who is Kitana's (Talisa Soto) long-dead mother. Earthrealm is therefore in danger of being absorbed into Outworld within six days, a fate which Liu Kang (Robin Shou) and the others must fight to prevent. Kahn fights and quickly kills Johnny Cage (Chris Conrad) during the confrontation by snapping his neck, and the remaining Earthrealm warriors must regroup and think of an idea to defeat Shao Kahn. An emotionally guilt-ridden Sonya (Sandra Hess) enlists the help of her old partner, Jax (Lynn "Red" Williams), while Kitana and Liu Kang search for a Native American shaman named Nightwolf (Litefoot), who seemingly knows the key to defeating Kahn. On the way, they run afoul of the cyborg Smoke. Kitana and Liu Kang dispatch him with the aid of Sub-Zero (Keith Cooke), but Scorpion (J.J. Perry) suddenly appears, attacks Sub-Zero, and kidnaps Kitana.Raiden (James Remar) meets with the Elder Gods and asks them why Kahn was allowed to break the tournament rules and force his way into Earthrealm, and how he can be stopped. The answers he receives are sparse and ambiguous; one says that reuniting Kitana with her mother, Sindel, is the key to breaking Kahn's hold on Earthrealm, but another Elder God insists that the defeat of Kahn himself is the solution. Raiden is then asked by the Elder Gods about his feelings and obligations towards the mortals, and what he would be willing to do to ensure their survival. Liu Kang finds Nightwolf, who teaches him about the power of the Animality, a form of shapeshifting which utilizes the caster's strengths and abilities. To achieve the mindset needed to acquire this power, Liu Kang must pass three tests. The first is a trial of his self-esteem and focus. The second comes in the form of temptation, which manifests itself in the form of Jade (Irina Pantaeva), who attempts to seduce Liu Kang and make him forget about Kitana. Liu Kang resists Jade's advances, which impresses her. She offers her assistance in fighting Kahn. Liu Kang accepts Jade's offer and takes her with him to the Elder Gods' temple, where he and his friends are to meet Raiden. The third test is never seen.At the temple, the Earthrealm warriors reunite with a newly shorn Raiden, who explains that he has sacrificed his immortality to freely fight alongside them. Together, they head for Outworld to rescue Kitana and reunite her with Sindel. With Jade's help, Liu Kang sneaks into Kahn's castle and rescues Kitana, while the others find Sindel. Unfortunately, Sindel remains under Kahn's control, and she escapes while a trio of Raptors ambush the heroes, while Jade reveals herself to be a mole sent by Kahn to disrupt the heroes' plans. Raiden then reveals that Shao Kahn is his brother, and that Elder God Shinnok (Reiner Schöne) is their father. He realizes that Shinnok had lied to him and is supporting Kahn. With renewed purpose, Raiden and the Earthrealm warriors make their way to the final showdown with Kahn and his generals. Shinnok demands that Raiden submit to him and restore their broken family, at the expense of his mortal friends. Raiden refuses and is killed by an energy blast from Shao Kahn.Though the early going is rough, Jax, Sonya, and Kitana emerge victorious against their opponents, but Liu Kang struggles with Kahn, and his Animality barely proves effective. Shinnok attempts to intervene and kill Liu Kang on Kahn's behalf, but two of the Elder Gods arrive, having uncovered Shinnok's treachery. They declare that the fate of Earth shall be decided in Mortal Kombat. Liu Kang finally defeats Kahn, and Shinnok is banished to the Netherrealm. Earthrealm reverts to its former state, and with Kahn's hold over Sindel finally broken, she reunites with Kitana. Raiden is revived by the other Elder Gods, who bestow upon him his father's former position. With everything right in the universe once again, the Earthrealm heroes return home triumphant.
- Ash Ketchum, Pikachu, Misty, Togepi, Brock and the Pokemon gang, Simba, Nala, Timon, Pumbaa, Zazu, Kovu, Kiara, Ma, Uncle Max, Rafiki, Littlefoot, Cera, Ducky, Petrie, Spike, Chomper, Ruby, Guido, Grandpa Longneck, Grandma Longneck and the Land Before Time Gang, SpongeBob SquarePants, Patrick Star, Squidward Tentacles, Sandy Cheeks, Eugene Krabs and the gang, Bloom, Stella, Flora, Musa, Tecna, Layla, Stan Marsh, Kyle Broflovski, Eric Cartman, Kenny McCormick, Jean-Bob, Speed, Puffin, Terk, Tantor, Louis, Ray the Firefly, Genie, Iago, Sebastian, Baloo, Bagheera, King Louie, the Vultures, Jiminy Cricket, Mickey Mouse, Donald Duck, Goofy, Kenai, Koda, Rutt and Tuke, Fu Dog, Roger Rabbit, Woody, Buzz Lightyear, Charlie Barkin, Itchy Itchiford, Jack Skellington, Chanticleer, Owen, Izzy, Bowser, Mistress Nine, Bowser Jr., Ranamon, Oogie Boogie, Bob the Tomato, Larry the Cucumber, Junior Asparagus, Jimmy and Jerry Gourd, the Powerpuff Girls (Blossom, Bubbles, and Buttercup), the Rugrats Gang, Carly Shay, Sam Puckett, Alvin, Brittany, Bobby, Cindy, Oliver, Freddie Benson, Spencer Shay, Gibby, the Muppets (Kermit the Frog, Miss Piggy, Fozzie Bear, Gonzo, Rizzo the Rat, Waldorf and Statler, Sam the Eagle, Animal, and the Electric Mayhem), Popeye, Olive Oyl, Popeye's Nephews (Peepeye, Poopeye, Pipeye, and Pupeye), Scrooge McDuck, Huey, Dewey, Louie, Webby, Launchpad McQuack, Chip and Dale, Lilo, Stitch, Pleakey, Jumba, Mario, Luigi, Princess Toadstool, Toad, Yoshi, Donkey Kong, Diddy Kong, Sonic the Hedgehog, Miles "Tails" Prower, Knuckles the Echidna, Amy Rose, Cream the Rabbit, Cheese the Chao, Shadow the Hedgehog, Rouge the Bat, Team Chaotix (Vector the Crocodile, Espio the Chameleon, and Charmy the Bee), Silver the Hedgehog, Blaze the Cat, Spyro the Dragon, Sparx the Dragonfly, Banjo and Kazooie, Barney the Dinosaur, Baby Bop, BJ, Riff, The Griffins (Peter, Lois, Chirs, Meg, Brian, and Stewie), Pocahontas, Meeko, Flit, Princess Aurora, Peter Pan, Tinkerbell, Terrence, Fawn, Rosetta, Silvermist, Iridessa, Vidia, Alice, Hercules, Phlioctetes, Pegasus, Phineas and Ferb, Isabella Garcia-Shapiro, Alex, Marty, Melman, Gloria, The Penguins of Madagscar (Skipper, Kolwalski, Rico, and Private), King Julien, Maurice, Mort, Taran, Princess Eilonwy, Fflewddur Fflam, Gurgi, Jesse Greenwood, Elvis, Randolph Johnson, Nadine, The Goonies (Mikey Walsh, Brand Walsh, Mouth Devereaux, Data Wang, Chunk Cohen, Andy Carmichael, Stef Steinbrenner, and Sloth Fratelli), Aladdin, Princess Jasmine, Abu, Yogi Bear, Boo-Boo Bear, Snagglepuss, Huckleberry Hound, Quick Draw McGraw, Augie Doggie and Doggie Daddy, Wally Gator, Magilla Gorilla, Top Cat, Peter Potamus, Scrooge McDuck, Huey, Dewey and Louie, Webby, Tom, Huckleberry, Becky, Scooby-Doo, Shaggy Rogers, Fred Jones, Daphne Blake, Velma Dinkley, Bugs Bunny, Daffy Duck, Woody Woodpecker, Ttark, Rex, Woog, Elsa, Dweeb, Fievel Mousekewitz, Tiger, Tony Toponi, Tanya Mousekewitz, Yasha Mousekewitz, Papa Mousekewitz, Mama Mousekewitz, Kids World's Adventures Team, Hubie, Marina, Rocko, Makunga, Creeper, The Machine, The Fratellis (Ma Fratelli, Francis Fratelli, and Jake Fratelli), Abis Mal, Dick Dastardly, Muttley, Injun Joe, Flintheart Glomgold, The Beagle Boys, Drake, Warren T. Rat, and Cat R. Waulthe Queen of Hearts, Dr. Eggman, Mojo Jojo, Him, Fuzzy Lumpkins, the Gang Green Gang, the Scallions, the Bad Apple, Carface, Killer, Scar, Shenzi, Banzai, Ed, Emperor Zurg, Jafar, Maleficent, Myotismon, Lord Rothbart, Shere Khan, Cruella De Vil, Ursula, Clayton, Pete, Dr. Facilier, the Grand Duke of Owls, Syndrome, Fat Cat, Bowser,Mistress Nine Bowser Jr., King K. Rool, Dr. Ivo "Eggman" Robotnik, Scratch and Grounder, General Specific, and Private Public, Sheldon J. Plankton, Heather, Ichy and Dil,, Mojo Jojo, Ojo Tango, Rocko Socko, Baboon Kaboom, the Gogo Patrol, Hota Wata, Cha-Ching Cha-Ching, the Doot Da Doot Da Doo Doos, Hacha Chacha, Blah-Blah Blah-Blah, Killa Drilla, Bonzo Bango, Rolo Ovo, Cruncha Muncha, Wacko Smocko, Pappy Wappy, Him, the Rowdyruff Boys, Fuzzy Lumpkins, the Gang Green Gang, the Scallions, the Bad Apple, Team Rocket (Jessie, James, and Meowth), Jafar, Iago, Maleficent, Dr. Facilier, Ursula, Captain Hook, Vidia, the Evil Queen, Pete, the Big Bad Wolf, Hades, Pain and Panic, Dr. Eggman, Scratch and Grounder, Emperor Zurg, Lotso the Bear, Nigel the Cockatoo, Mojo Jojo, the Gang Green Gang, Him, Fuzzy Lumpkins, Crud, Nasty Jack, the Moopets, and the bad guys will guest star in this film.