Ryan, Winnie the Pooh, Ash, Michelle and the rest of their friends meet Alex Pearson (Hutch Dano) is an avid Lemon Oaks High School hockey player who is seeking the attention of Matisse Burrows (Kelsey Chow), the most attractive girl in school. All he wants is a car, but his father refuses to buy him one until his 'attitude' improves. After showing off at one of his games and getting suspended from the team, Alex's chances of getting a car majorly decreases. In order to get the car, he has to do chores and babysit his little sister, Emily (G. Hannelius). He unexpectedly becomes the new unofficial leader of Emily's Bumble Bee troop while masquerading under the alias Mrs. Zamboni. The Bumble Bee troop has to work to be able to attend the Camporee, the most important Bumble Bee event of the year, by selling cookies and completing various badges. Originally the badges are made up by Alex for doing his chores, but after it gets them disqualified, Alex helps them get real badges. At the same time, he also stands up for the other Den Mothers to Dina (Vicki Lewis), a very controlling Den Mother. The Bumble Bees and Alex finally earn enough to attend the event. Alex accidentally reveals himself when, during a muffin sale at the big hockey game, he puts himself in, still disguised as Mrs. Zamboni, to win the game. Doing so disqualifies his troop, alienates his best friend, and disappoints his sister and father. Afterwards, Emily refuses to talk to him and his father refuses to punish him, knowing that Alex will punish himself. After returning his neighbor's dog, Alex learns that she used to be a Bumble Bee herself and that his mother, who had died two years ago, was in her daughter's troop. Upon learning this, Alex makes a deal with her to help out around the house in exchange for her being the new Den Mother. After introducing her to the troop, she convinces them to forgive Alex, who goes to the finals for his hockey game. There, he apologizes to his team and offers captain to Goose (David Lambert), who refuses, saying he wants Alex to be the real captain. During the game, Alex acts as a distraction instead of hogging the puck to himself, allowing his team to win. He sacrifices the winning shot to Goose, causing Goose to get the All-Star spot Alex wanted. The Bumble Bees show up with Alex's father; Matisse forgives Alex and reveals that they want Alex to be their Den Mother, to which Alex accepts. At the Camporee, Alex is forced to put on the Bumble Bee uniform in order to be the Den Mother. Dina, his rival, sends her husband to put a stop to what he's doing, but instead, the man, who was the referee responsible for Alex's suspension, is impressed by Alex and offers him a spot on the All Star team. Alex accepts as long as it works around his troop's schedule with Ryan, Winnie the Pooh, Ash Ketchum, Littlefoot, Spongebob, Skipper, The Fantasy Adventure Team, Bobby, Cindy, Greg, Marcia, Peter, Jan, Oliver, Millicent, DJ, Stephanie, Michelle, Alex, Nicky, Malcolm, Mallory, Reese, Renee, Dewey, Daisy, Emily Yeung, Daniel Cook, the Jungle Adventure Crew, the Madagascar Crew, the cubs, Aladar, Taran, Jesse, Mikey, Dennis Mitchell, Bloom, The characters of Diary of a Wimpy Kid, Michael, the rest of their friends.
- Bobby, Cindy, Oliver, Matthew, Dwayne, Steve, DJ, Stephanie, Michelle, Malcolm, Resse, Dewey, Mallory, Renee, Daisy, Gabriella, Gerald, May, Sid, Kady, Franklin and The Gang, Alvin, Simon, Theodore, Brittany, Jeanette, Elanor, Ben Hilton, Jamie, Max, Becca, Nicky, Jeannie, The Flash/Tony, Crispin, Dylan, Duncan, Sean, Tony's Brother, German Speaking Boy, Juel, Erin, Benny, Lina, Henry, Kiki, Dennis The Menace & Friends, Pete & Pete, Richelle, Littlefoot, Cera, Ducky, Petrie, Spike, SpongeBob SquarePants, Patrick Star, Bloom, Stella, Flora, Musa, Tecna, Layla, Roxy, Simba, Timon, Pumbaa, Zazu, Terk, Tantor, Baloo, Bagheera, King Louie, the Vultures (Buzzie, Flaps, Ziggy, and Dizzy), Jiminy Cricket, Alex, Marty, Melman, Gloria, the Penguins of Madagascar (Skipper, Kolwalski, Rico, and Private), Genie, Louis, Roger Rabbit, Danny, Sawyer, Rex, Dweeb, Woog, Esla, Rapunzel, Pascal, Flynn Rider, Maximus, Jean-Bob, Speed, Puffin, Chanticleer, Wilbur the Albatross, Owen, Lindsay, Beth, Strawberry Shortcake and her friends, Danny Phantom, Sam Manson, Tucker Foley, Jazz Fenton, Danny, Sawyer, Jean-Bob, Speed, Buddy, Tiny, Puffin, Rex, Dweeb, Woog, Elsa, Rapunzel, Pascal, Flynn Rider, Maximus, Chanticleer, Wilbur the Albatross, Bob the Tomato, Larry the Cucumber, Junior Asparagus, Hercules, Megara, Philoctetes, Pegasus, Alice, Pocahontas, Meeko, Flit, Princess Aurora, Prince Phillip, Peter Pan, Tinkerbell, Rosetta, Silvermist, Idressa, Fawn, Oliver, Dodger, Lucky, Cadpig, Rolly, Spot the Chicken, Woody, Buzz Lightyear, Jessie, Tommy Pickles, Chuckie Finster, Simon, Theodore, Brittany, Huckle, Lowly, Woody Woodpecker, Ord, Cassie, Arthur Read Ash and his friends, Ronald McDonald and his friends, Bob the Tomato, Larry the Cucumber, Junior Asparagus, Laura Carrot, Jimmy and Jerry Gourd, Mr. Nezzer, Mr. Lunt, the Powerpuff Girls (Blossom, Bubbles, and Buttercup), Professor Utonium, Miss Keane, Blue the Puppy, Ploka Dots, Slippery Soap, Tickety Tock, Mr. Salt, Mrs. Pepper, Paprika, Periwinkle, Roar E. Saurus, Fredrica the Birthday Girl, Zazu, Rafiki, Kovu, Kiara, Terk, Tantor, the All-Grown Up Gang, Grandpa Lou Pickles, Carly Shay, Sam Puckett, Freddie Benson, Spencer Shay, Gibby, The Gummi Bears, Scrooge McDuck, Huey, Dewey, Louie, Webby, Launchpad McQuack, Darkwing Duck, Gosalyn Mallard, Honker Muddlefoot, Chip and Dale, Gadget Hackwrench, Monterey Jack, Zipper, Lilo, Stitch, Pleakey, Jumba, the Muppets (Kermit the Frog, Miss Piggy, Fozzie Bear, Gonzo, Bean Bunny, Rowlf the Dog, Scooter, Rizzo the Rat, Waldorf and Statler, Pepe the King Prawn, Sweetums, Thog, Sam the Eagle, the Electric Mayhem and Walter), Popeye, Olive Oyl, Popeye's Nephews (Peepeye, Poopeye, Pipeye, and Pupeye), Wimpy, Mario, Luigi, Princess Toadstool, Toad, Yoshi, Donkey Kong, Diddy Kong, Sonic the Hedgehog, Miles "Tails" Prower, Knuckles the Echidna, Amy Rose, Cream the Rabbit, Cheese the Chao, Shadow the Hedgehog, Rouge the Bat, Team Chaotix (Vector the Crocodile, Espio the Chameleon, and Charmy the Bee), Silver the Hedgehog, Blaze the Cat, Spyro the Dragon, Sparx the Dragonfly, Banjo and Kazooie, The Griffins (Peter, Lois, Chris, Meg, Brian, and Stewie), Glenn Quagmire, Cleveland Brown, The Teenage Mutant Ninja Turtles, Harry Potter, Hermoine Granger, Ron Weasley, Anastasia, Alex, Marty, Melman, Gloria, the Penguins of Madagascar (Skipper, Kowalski, Rico, and Private), King Julien, Maurice, Mort, Vitaly, Gia, Stefano, Marlene, Baloo, Bagheera, King Louie, Kit Cloudkicker, Rebecca Cunningham, the Vultures, Pinocchio, Jiminy Cricket, Geppetto, Woody, Buzz Lightyear, Jessie, Hamm, Slinky Dog, Rex, Mr. Potato Head, Mrs. Potato Head, the Squeeze Toy Aliens, Bullseye, Dolly, Mr. Pricklepants, Buttercup, Trixie, Chuckles the Clown, the Incredibles (Bob, Helen, Dash, Violet, Jack-Jack, and Frozone), Cinderella, Snow White, Ariel, Sebastian, Belle, Lumiere, Cogsworth, Mrs. Potts, Chip the Cup, Aladdin, Princess Jasmine, Genie, Abu, Mulan, Mushu, Cri-Kee, Pocahontas, Meeko, Flit, Princess Aurora, Princess Tiana, Louis, Rapunzel, Flynn Rider, Pascal, Maximus, Princess Merida, Peter Pan, Tinker Bell, Terence, Fawn, Rosetta, Silvermist, Iridessa, Vidia, Lucky, Cadpig, Rolly, Spot the Chicken, Alice, Hercules, Megara, Phlioctetes, Pegasus, Phineas and Ferb, Candace Flynn, Isabella Garcia-Shapiro, Stacy Hirano, Buford, Baljeet, Perry the Platypus, Chip, Dale, Baby Miss Piggy, the Regular Show Gang, Pajama Sam, Ash and the characters, Littlefoot and the characters, the Madagascar gang, C.G. and her Friends, the Kids Next Door, Numbuh 362, Iago, Bloom and her friends, Ace Bunny and his friends, Scooby-Doo and the gang, The Flintstones gang, The Jetsons gang, Yogi Bear and the characters, the vultures, Jimmy Cricket Zazu, Kronk, the Tiny Toons Gang, Ronald Mcdonald and his Friends, Ttark, Earthworm Jim, Peter Puppy, Clifford the big red dog and his friends, Dink the Little Dinosaur and his friends, Spyro the dragon and his friends, Charlie Brown and the characters, Thomas the tank engine and his friends, Toucan Sam and his nephews, Tony the tiger, Sailor Moon and the Sailor scouts, Cap'n Crunch, Mike, Sulley, Gumby and the characters and the rest of their cartoon characters of heores, Kids World's Adventures Team, and the characters will guest star in this film.